PDCD4 Antikörper (pSer457)

Produktnummer ABIN214444

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PDCD4 in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für PDCD4 Antikörper (pSer457) (ABIN214444)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDCD4 Antikörper

Bindungsspezifität: pSer457

Spezifität: Amino acids 447-465 of human Pdcd4 protein.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human PDCD4.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1500 - 1:7000), IHC, IHC-P (1.25 - 2.5 μg/mL), WB (1:200 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25-2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4

Hintergrund: Name/Gene ID: PDCD4
Family: Apoptosis

Synonyms: PDCD4, H731, Nuclear antigen H731-like, Nuclear antigen H731, Protein 197/15a

Gen-ID: 27250

UniProt: Q53EL6