Aurora Kinase B Antikörper (AA 227-238)

Produktnummer ABIN214031

Der Kaninchen Polyklonal anti-Aurora Kinase B Antikörper wird verwendet zum Nachweis von Aurora Kinase B in Proben von Human. Er wurde validiert für IHC, WB, ELISA, IF und IHC (p).

Kurzübersicht für Aurora Kinase B Antikörper (AA 227-238) (ABIN214031)

Target: Aurora Kinase B (AURKB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: AA 227-238

Spezifität: Aa 227-238 of Human Aurora Kinase B protein.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human AURKB / Aurora-B.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: AURKB / Aurora-B

Hintergrund: Name/Gene ID: AURKB
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKB, AIRK2, AIK2, Aurora kinase B, Aurora kinase B-Sv1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, ARK-2, ARK2, Aurkb-sv1, Aurkb-sv2, Aurora 1, Aurora-B, AIM-1, IPL1, STK-1, STK5, PPP1R48, AurB, Aurora B, Aurora kinase B-Sv2, Aurora-1, Aurora1, Serine/threonine kinase 12, STK1, STK12

Gen-ID: 9212

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades