PUMA Antikörper (C-Term)

Produktnummer ABIN213906

Dieses Anti-PUMA-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PUMA in WB, IHC, ICC und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für PUMA Antikörper (C-Term) (ABIN213906)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.

Aufreinigung: Immunoaffinity purified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: PUMA

Hintergrund: Name/Gene ID: BBC3
Family: Apoptosis

Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA

Gen-ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity