NEK2 Antikörper (AA 287-299)

Produktnummer ABIN213864

Produktdetails anti-NEK2 Antikörper

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Bindungsspezifität: AA 287-299

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Aa 287-299 of Human NEK2 protein.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human NEK2.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Details zu NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Andere Bezeichnung: NEK2 (NEK2 Produkte)

Synonyme: AA617254 antikoerper, C77054 antikoerper, HsPK21 antikoerper, NEK2A antikoerper, NLK1 antikoerper, ATNEK2 antikoerper, NIMA-RELATED KINASE2 antikoerper, NIMA-related kinase 2 antikoerper, T9J14.24 antikoerper, T9J14_24 antikoerper, NIMA related kinase 2 antikoerper, NIMA (never in mitosis gene a)-related expressed kinase 2 antikoerper, NIMA-related kinase 2 antikoerper, NEK2 antikoerper, Nek2 antikoerper

Hintergrund: Name/Gene ID: NEK2
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2

Gen-ID: 4751

UniProt: P51955

Pathways: M Phase