COL2 Antikörper

Produktnummer ABIN2137989

Der Kaninchen Polyklonal Anti-COL2-Antikörper wurde für WB, IHC, ELISA und IP validiert. Er ist geeignet, COL2 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für COL2 Antikörper (ABIN2137989)

Target: COL2 (Collagen, Type II (COL2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktdetails anti-COL2 Antikörper

Spezifität: This lot of antisera reacts to both human and bovine PTH C-Terminal region.

Kreuzreaktivität: Ratte (Rattus), Maus

Kreuzreaktivität (Details): This antibody that has been prepared bi immunoaffininty chromotography using immobilized antigens is followed by extensive cross-absorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins toremove any unwanted specifities.

Aufreinigung: Purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: Collagen type II antibody was raised in rabbit using collagen type II from human knee cartilage and bovine nasal cartilage as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1:5,000-1:10,000, IHC: 1:50-1,200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Kommentare:

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Sterile filtered liquid In 0.125 M Na2B4O7, pH 8.0, with 0.075 M NaCl, 0.005 M EDTA, and 0.01 % Sodium azide.

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Referenzen für anti-COL2 Antikörper (ABIN2137989)

Murphy, Huey, Hu, Athanasiou: "TGF-β1, GDF-5, and BMP-2 stimulation induces chondrogenesis in expanded human articular chondrocytes and marrow-derived stromal cells." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 3, pp. 762-73, (2015) (PubMed).

Details zu COL2

Target: COL2 (Collagen, Type II (COL2))

Andere Bezeichnung: Collagen Type II