ERBB3 Antikörper (C-Term)

Produktnummer ABIN213195

Dieses Anti-ERBB3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ERBB3 in WB und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ERBB3 Antikörper (C-Term) (ABIN213195)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERBB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-ERBB3 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes human HER3 at 180kD. Species cross-reactivity: mouse and rat. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum.

Aufreinigung: Protein A purified

Immunogen: A GST-HER3 fusion protein corresponding to aa1283 to 1323 at the carboxy-terminus of human HER3.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 5 μL. Use 25 μL of Protein A-agarose beads and 1 mL of lysate (lysate contains approximately 1 mg of total protein). Rotate mixture overnight at 4?C and then wash 3x with lysis buffer. Dissolve the resulting bead complex in 20-30 μL of 3xSDS-PAGE sample buffer and load ~15 μL per lane on an 8 % polyacrylamide gel. Positive control: Human prostate cell lines (LNCap) or human breast cell line (MDA-453).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: HER3 / ERBB3

Hintergrund: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

Gen-ID: 2065

UniProt: P21860

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung