EPH Receptor A2 Antikörper

Produktnummer ABIN211915

Dieses Maus Monoklonal-Antikörper erkennt spezifisch EPH Receptor A2 in WB, IP, ICC und Func. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Rind (Kuh).

Kurzübersicht für EPH Receptor A2 Antikörper (ABIN211915)

Target: EPH Receptor A2 (EPHA2)

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPH Receptor A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Produktdetails anti-EPH Receptor A2 Antikörper

Spezifität: Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.

Aufreinigung: Protein G purified

Immunogen: Native protein isolated by purification of phosphotyrosine-containing proteins.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Func, ICC, IP, WB

Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: EPHA2 / EPH Receptor A2

Hintergrund: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

Gen-ID: 1969

UniProt: P29317

Pathways: RTK Signalweg