Vinculin Antikörper

Produktnummer ABIN211429

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Vinculin in WB, IHC, IP, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Kaninchen und Huhn.

Kurzübersicht für Vinculin Antikörper (ABIN211429)

Target: Vinculin (VCL)

Reaktivität: Human, Maus, Ratte, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Vinculin Antikörper

Spezifität: Recognizes human vinculin at 117kD. Species cross-reactivity: rat, mouse, rabbit and chicken.

Aufreinigung: Protein A purified

Immunogen: Human vinculin.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-Fr, IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10 μg/mL will stain Vinculin in Ref-52 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: VCL / Vinculin

Hintergrund: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gen-ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2