SMC1A Antikörper (pSer957)

Produktnummer ABIN211403

Der Maus Monoklonal Anti-SMC1A-Antikörper wurde für WB validiert. Er ist geeignet, SMC1A in Proben von Human, Rind (Kuh), Hund, Kaninchen und Affe zu detektieren.

Kurzübersicht für SMC1A Antikörper (pSer957) (ABIN211403)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reaktivität: Human, Rind (Kuh), Hund, Kaninchen, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMC1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SMC1A Antikörper

Bindungsspezifität: AA 951-962, pSer957

Spezifität: Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.

Aufreinigung: Protein G purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 1-2 μg/mL detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Andere Bezeichnung: SMC1A / SMC1

Hintergrund: Name/Gene ID: SMC1A

Synonyms: SMC1A, CDLS2, DXS423E, KIAA0178, SB1.8, SMC protein 1A, SMC1, SMC1L1, SMC-1-alpha, SMC-1A, SMCB

Gen-ID: 8243

UniProt: Q14683

Pathways: Stem Cell Maintenance