Nibrin Antikörper (AA 420-774)

Produktnummer ABIN210930

Dieses Anti-Nibrin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nibrin in WB und IP. Geeignet für Human.

Kurzübersicht für Nibrin Antikörper (AA 420-774) (ABIN210930)

Target: Nibrin (NBN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nibrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-Nibrin Antikörper

Bindungsspezifität: AA 420-774

Spezifität: Recognizes human Nibrin. Recognizes Nibrin/Nbs1, Mr 95kD. A non-specific protein was also detected at Mr 33kD in some lysates, but not Jurkat cell lysate. Species cross-reactivity: Human, NOT mouse.

Aufreinigung: Protein G purified

Immunogen: GST fusion protein corresponding to residues 420-774 of human Nibrin.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitates Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Nibrin

Target: Nibrin (NBN)

Andere Bezeichnung: NBN / NBS1 / Nibrin

Hintergrund: Name/Gene ID: NBN

Synonyms: NBN, ATV, AT-V2, Nibrin, NBS1, AT-V1, NBS, p95

Gen-ID: 4683

UniProt: O60934

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response