GRIK1 Antikörper (AA 937-949)

Produktnummer ABIN210888

Produktdetails anti-GRIK1 Antikörper

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Bindungsspezifität: AA 937-949

Reaktivität: Human, Ratte, Maus, Kaninchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRIK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Recognizes GluR5 isoforms, Mr 105-110kD. Species cross-reactivity: Rat. Predicted to cross-react with certain human and mouse GluR5 isoforms, based on sequence homology.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit (100%) Bovine, Horse (92%) Chicken, Lizard (85%).

Aufreinigung: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 937-949 (CHQRRTQRKETVA) of rat GluR5-2C isoform precursor. This sequence is identical in rat isoforms GluR5-2 and GluR5-2B/GluR5-1 and human GluR5 isoform 2/EAA3A, and is not present in rat isoform GluR5-2A and human GluR5 isoform 1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Rabbit (100%), Bovine, Horse (92%), Chicken, Lizard (85%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Recommended Dilution: Western Blot: 0.5-2 μg/mL detects GluR5 in RIPA lysates from a rat brain microsomal preparation. Western Blot Analysis: Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-GluR5 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Andere Bezeichnung: GRIK1 / GLUR5 (GRIK1 Produkte)

Hintergrund: Name/Gene ID: GRIK1
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIK1, GluR-5, GLUR5, EEA3, GluK1, Glutamate receptor 5, EAA3, GLR5

Gen-ID: 2897

UniProt: P39086

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity