MMP14 Antikörper (AA 319-333)
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- Target Alle MMP14 Antikörper anzeigen
- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
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Bindungsspezifität
- AA 319-333
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Reaktivität
- Human, Maus, Ratte, Rind (Kuh), Affe, Ziege, Hamster, Schaf
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser MMP14 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
- Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).
- Isotyp
- IgG3 kappa
- Top Product
- Discover our top product MMP14 Primärantikörper
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- Applikationshinweise
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Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, pH 7.0, 2 % BSA (protease free).
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
- Andere Bezeichnung
- MMP14 / MMP-14 (MMP14 Produkte)
- Synonyme
- MMP1 antikoerper, MMP-14 antikoerper, MMP-X1 antikoerper, MT-MMP antikoerper, MT-MMP 1 antikoerper, MT1-MMP antikoerper, MT1MMP antikoerper, MTMMP1 antikoerper, mmp-x1 antikoerper, mt1-mmp antikoerper, mtmmp1 antikoerper, AI325305 antikoerper, MT-MMP-1 antikoerper, sabe antikoerper, Mt1-mmp antikoerper, MT-MMPbeta antikoerper, cb386 antikoerper, sb:cb386 antikoerper, wu:fb72b09 antikoerper, mmp14 antikoerper, matrix metallopeptidase 14 antikoerper, matrix metallopeptidase 14 (membrane-inserted) antikoerper, matrix metallopeptidase 14b (membrane-inserted) antikoerper, matrix metallopeptidase 14a (membrane-inserted) antikoerper, matrix metallopeptidase 14 L homeolog antikoerper, MMP14 antikoerper, mmp14 antikoerper, Mmp14 antikoerper, mmp14b antikoerper, mmp14a antikoerper, mmp14.L antikoerper
- Hintergrund
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Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease
Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1 - Gen-ID
- 4323
- UniProt
- P50281
- Pathways
- Autophagie
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