MMP14 Antikörper (AA 319-333)

Produktnummer ABIN209030

Der Maus Monoklonal Anti-MMP14-Antikörper wurde für WB, ELISA, IHC, IP, IHC (p) und IHC (fro) validiert. Er ist geeignet, MMP14 in Proben von Human, Maus, Ratte, Rind (Kuh), Affe, Ziege, Hamster und Schaf zu detektieren.

Kurzübersicht für MMP14 Antikörper (AA 319-333) (ABIN209030)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Affe, Ziege, Hamster, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MMP14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MMP14 Antikörper

Bindungsspezifität: AA 319-333

Spezifität: Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).

Isotyp: IgG3 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.0, 2 % BSA (protease free).

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Andere Bezeichnung: MMP14 / MMP-14

Hintergrund: Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1

Gen-ID: 4323

UniProt: P50281

Pathways: Autophagie