IRS1 Antikörper (AA 1222-1235)

Produktnummer ABIN208882

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IRS1 in WB und IP. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für IRS1 Antikörper (AA 1222-1235) (ABIN208882)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-IRS1 Antikörper

Bindungsspezifität: AA 1222-1235

Spezifität: Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.

Homologie: Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the carboxy terminal of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/mL BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS1 / IRS-1

Hintergrund: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

Gen-ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process