H2AFX Antikörper (pSer139)

Produktnummer ABIN208587

Dieser Anti-H2AFX-Antikörper ist ein Maus-Monoklonal-Antikörper zum Nachweis von H2AFX in WB, IF, ICC und IP. Geeignet für Human, Rind (Kuh), Hund, Kaninchen und Affe.

Kurzübersicht für H2AFX Antikörper (pSer139) (ABIN208587)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human, Rind (Kuh), Hund, Kaninchen, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser H2AFX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-H2AFX Antikörper

Bindungsspezifität: AA 134-142, pSer139

Spezifität: Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC (2 μg/mL), IF, IP, WB

Usage: Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 μg/mL detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: H2AX

Hintergrund: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

Gen-ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus