DNMT3L Antikörper (AA 152-164)
Kurzübersicht für DNMT3L Antikörper (AA 152-164) (ABIN208362)
Target
Alle DNMT3L (TRDMT1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 152-164
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Spezifität
- Recognizes Human DNMT3L MW: 43.6kD. Species sequence Homology: Mouse: 76 % .
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Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey (92%) Rat, Hamster, Panda, Bat (85%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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A synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey (92%), Rat, Hamster, Panda, Bat (85%).
Type of Immunogen: Synthetic peptide
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Applikationshinweise
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Approved: ELISA (1:1000 - 1:3000), IP, WB (1:1500)
Usage: Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 1:1000-1:3000. Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells. (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150 mM. Does not perform well when applied directly for Western Blot. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
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Andere Bezeichnung
- DNMT3L
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Hintergrund
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Name/Gene ID: DNMT3L
Family: C5-methyltransferase
Synonyms: DNMT3L -
Gen-ID
- 29947
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UniProt
- Q9UJW3
Target
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