VCAM1 Antikörper

Produktnummer ABIN208158

Produktdetails anti-VCAM1 Antikörper

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VCAM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Reacts with human VCAM-1.

Aufreinigung: Purified

Immunogen:

Type of Immunogen: Native protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:10), IHC, IHC-Fr, IP

Usage: Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Andere Bezeichnung: VCAM1 / VCAM-1 / CD106 (VCAM1 Produkte)

Hintergrund: Name/Gene ID: VCAM1
Family: ICAM

Synonyms: VCAM1, CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1

Gen-ID: 7412

UniProt: P19320

Pathways: Carbohydrate Homeostasis