SOD2 Antikörper

Produktnummer ABIN204760

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SOD2 in WB, IHC, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für SOD2 Antikörper (ABIN204760)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SOD2 Antikörper

Spezifität: Recognizes human MnSOD. Species cross-reactivity: rat.

Aufreinigung: Protein G purified

Immunogen: Manganese superoxide dismutase (MnSOD).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-Fr (0.5 - 10 μg/mL), WB

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4 % PFA fixed frozen tissue. Typical block contains 1 % non-fat dry milk or 3 % NGS and 0.2-1.5 % triton X-100 (higher triton is only used on thick sections, blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk), typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4?C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites. Immunocytochemistry (cell smears): 0.5-10 μg/mL. Western Blot: Under reducing conditions a band at ~25kD will be identified.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Andere Bezeichnung: MNSOD / SOD2

Hintergrund: Name/Gene ID: SOD2

Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase

Gen-ID: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling