EPH Receptor A2 Antikörper (Extracellular Domain)

Produktnummer ABIN204192

Der Maus Monoklonal anti-EPH Receptor A2 Antikörper (ABIN204192) detektiert spezifisch EPH Receptor A2 in WB, FACS und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Hund und Rind (Kuh).

Kurzübersicht für EPH Receptor A2 Antikörper (Extracellular Domain) (ABIN204192)

Target: EPH Receptor A2 (EPHA2)

Reaktivität: Human, Maus, Hund, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPH Receptor A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-EPH Receptor A2 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes human EphA2.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rhEphA2 extracellular domain.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 200 μL sterile PBS.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handhabung: Avoid frequent freeze thaws. Store undiluted.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Details zu EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: EPHA2 / EPH Receptor A2

Hintergrund: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

Gen-ID: 1969

UniProt: P29317

Pathways: RTK Signalweg