GJA3 Antikörper (Internal Region)

Produktnummer ABIN200660

Der Kaninchen Polyklonal Anti-GJA3-Antikörper wurde für WB, IHC, IF und IHC (fro) validiert. Er ist geeignet, GJA3 in Proben von Maus zu detektieren.

Kurzübersicht für GJA3 Antikörper (Internal Region) (ABIN200660)

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GJA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-GJA3 Antikörper

Bindungsspezifität: Internal Region

Spezifität: Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IF (3 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~46kD. Immunofluorescence: 3 μg/mL. Note: For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin), followed by 40 mL fixative (0.16M sodium phosphate buffer,  pH 7.6, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer,  pH 7.4. Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer,  pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GJA3

Target: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Andere Bezeichnung: GJA3 / CX46 / Connexin 46

Hintergrund: Name/Gene ID: GJA3
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA3, Connexin-46, CX46, CZP3, Gap junction alpha-3 protein, Gap junction protein alpha 3, CAE3, Connexin 46

Gen-ID: 2700

UniProt: Q9Y6H8