PDGFRA Antikörper (Extracellular Domain)

Produktnummer ABIN198260

Der Maus Monoklonal Anti-PDGFRA-Antikörper wurde für WB, ELISA, IHC und IHC (p) validiert. Er ist geeignet, PDGFRA in Proben von Human zu detektieren.

Kurzübersicht für PDGFRA Antikörper (Extracellular Domain) (ABIN198260)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 35248 (57-20)

Produktdetails anti-PDGFRA Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kD single pass type I transmembrane glycoprotein. PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases. Has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

Aufreinigung: Protein G purified

Immunogen: Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC, IHC-P (10 - 20 μg/mL), WB (1 - 2 μg/mL)

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1.5 % Trehalose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: PDGFRA / PDGFR alpha

Hintergrund: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Gen-ID: 5156

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling