Phosphotyrosine Antikörper

Produktnummer ABIN197635

Dieser Maus Monoklonal Antikörper detektiert spezifisch Phosphotyrosine in WB, IF, IP, EIA und IHC (p).

Kurzübersicht für Phosphotyrosine Antikörper (ABIN197635)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PY20

Produktdetails anti-Phosphotyrosine Antikörper

Aufreinigung: Affinity Chromatography

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: ELISA (0.1 μg/mL)ImmunofluorescenceWestern blotting (2g/mL)Immmunoprecipitation: use Protein A and Ab at 2 g/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 5-10 μg/mL 30 min RT (Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min. followed by cooling at RT for 20 min. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Borate buffered saline pH 8.0, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 4 °C for up to one month or aliquot and store at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.