Der Kaninchen Polyklonal anti-RPS6 Antikörper wird verwendet zum Nachweis von RPS6 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IF.
This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at Serine 235.
Aufreinigung
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).
RPS6
Reaktivität: Maus, Ratte
WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
RPS6
(Ribosomal Protein S6 (RPS6))
Andere Bezeichnung
RPS6
Hintergrund
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33