FOXO4 Antikörper (pSer197)

Produktnummer ABIN196850

Der Kaninchen Polyklonal anti-FOXO4 Antikörper wird verwendet zum Nachweis von FOXO4 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für FOXO4 Antikörper (pSer197) (ABIN196850)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXO4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXO4 Antikörper

Bindungsspezifität: pSer197

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Andere Bezeichnung: FOXO4 / AFX1 / MLLT7

Hintergrund: FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.Synonyms: Fork head domain transcription factor AFX1, Forkhead box protein O4

Gen-ID: 4303

NCBI Accession: NP_001164402

UniProt: P98177

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation