LIM Domain Kinase 1 Antikörper (pThr508)

Produktnummer ABIN196830

Dieses Anti-LIM Domain Kinase 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LIM Domain Kinase 1 in WB, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für LIM Domain Kinase 1 Antikörper (pThr508) (ABIN196830)

Target: LIM Domain Kinase 1 (LIMK1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Bindungsspezifität: pThr508

Spezifität: This antibody detects endogenous levels of LIMK1 only when phosphorylated at Threonine 508.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK1

Hintergrund: LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. LIMK1 may be involved in brain development, it is highly expressed in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1

Gen-ID: 3984

NCBI Accession: NP_001191355

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events