Pyruvate Kinase, Muscle (PKM) Antikörper (Biotin)

Produktnummer ABIN1968075

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in WB und ELISA. Geeignet für Kaninchen.

Kurzübersicht für Pyruvate Kinase, Muscle (PKM) Antikörper (Biotin) (ABIN1968075)

Target: Pyruvate Kinase, Muscle (PKM)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

Aufreinigung: Ion exchange chromatography

Immunogen: Pyruvate Kinase [Rabbit Muscle].

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)

Usage: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Rabbit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handhabung: avoid freeze thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Antigendetails

Target: Pyruvate Kinase, Muscle (PKM)

Andere Bezeichnung: PKM / Pyruvate Kinase, Muscle

Hintergrund: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gen-ID: 5315

Pathways: Warburg Effekt