CXCR3 Antikörper

Produktnummer ABIN1967162

Produktdetails anti-CXCR3 Antikörper

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CXCR3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Neutralization (Neut), Functional Studies (Func)

Spezifität: Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.

Aufreinigung: Protein G purified

Immunogen: CXCR3 transfected NS0 cells.

Type of Immunogen: Cells

Klon: 2AR1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (5 - 10 μg/mL), Func, IHC (10 μg/mL), Neut

Usage: Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 μg/mL in the presence of 7 ng/mL of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 μg/mL, Add 10 μL to 1-2.5x10^5 cells in total reaction volume 200 μL. Visualize binding by adding 10 μL of 25 μg/mL IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 μg/mL detected CXCR3 in human tonsil. Cells were fixed in PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Cells were washed with PBS, BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 200 μL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Details zu CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Andere Bezeichnung: CXCR3 (CXCR3 Produkte)

Synonyme: CD182 antikoerper, CD183 antikoerper, CKR-L2 antikoerper, CMKAR3 antikoerper, GPR9 antikoerper, IP10-R antikoerper, Mig-R antikoerper, MigR antikoerper, Cd183 antikoerper, Cmkar3 antikoerper, CXCR3 antikoerper, cxcr3 antikoerper, Gpr9 antikoerper, CXC-R3 antikoerper, CXCR-3 antikoerper, zgc:92301 antikoerper, sb:eu378 antikoerper, C-X-C motif chemokine receptor 3 antikoerper, chemokine (C-X-C motif) receptor 3 antikoerper, chemokine (C-X-C motif) receptor 3, tandem duplicate 2 antikoerper, chemokine (C-X-C motif) receptor 3, tandem duplicate 1 antikoerper, CXCR3 antikoerper, Cxcr3 antikoerper, cxcr3 antikoerper, cxcr3.2 antikoerper, cxcr3.1 antikoerper

Hintergrund: Name/Gene ID: CXCR3
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR3, C-x-c chemokine receptor 3, CD183 antigen, CKR-L2, CXCR-3, CMKAR3, CD182, CD183, Cxcr3a, GPR9, IP-10 receptor, IP10-R, Mig receptor, MigR, Mig-R, Chemokine (C-X-C) receptor 3, CXC-R3, Cxcr3b, G protein-coupled receptor 9, IP10 receptor

Gen-ID: 2833

UniProt: P49682