Nibrin Antikörper (pSer343)

Produktnummer ABIN196714

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Nibrin in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Nibrin Antikörper (pSer343) (ABIN196714)

Target: Nibrin (NBN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nibrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Nibrin Antikörper

Bindungsspezifität: pSer343

Spezifität: The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. This antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Serine 343.

Aufreinigung: Affinity Chromatography using epitope-specific peptide

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p95/NBS1 around the phosphorylation site of Serine 343 (S-L-Sp-Q-G).

Anwendungsinformationen

Applikationshinweise: Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Nibrin

Target: Nibrin (NBN)

Andere Bezeichnung: Nibrin

Hintergrund: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95

Gen-ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response