FEN1 Antikörper

Produktnummer ABIN1944939

Dieses Anti-FEN1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FEN1 in WB, IHC und IF. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für FEN1 Antikörper (ABIN1944939)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-FEN1 Antikörper

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF: 1:100. WB: 1:1000. IHC: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C,-20 °C

Referenzen für anti-FEN1 Antikörper (ABIN1944939)

Taylor, Noguchi, Totoki, Toyoda, Kuroki, Dewar, Lloyd, Itoh, Takeda, Kim, She, Barlow, Bloom, Bruford, Chang, Cuomo, Eichler, FitzGerald, Jaffe, LaButti, Nicol, Park, Seaman, Sougnez, Yang, Zimmer et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification. ..." in: Nature, Vol. 440, Issue 7083, pp. 497-500, (2006) (PubMed).

Shen, Nolan, Sklar, Park: "Essential amino acids for substrate binding and catalysis of human flap endonuclease 1." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9173-6, (1996) (PubMed).

Murray, Tavassoli, al-Harithy, Sheldrick, Lehmann, Carr, Watts: "Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4878-88, (1994) (PubMed).

Robins, Pappin, Wood, Lindahl: "Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I." in: The Journal of biological chemistry, Vol. 269, Issue 46, pp. 28535-8, (1994) (PubMed).

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: FEN1

Hintergrund: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Molekulargewicht: 42593 Da

Gen-ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA