ATG4D Antikörper (C-Term)

Produktnummer ABIN1944848

Der Kaninchen Polyklonal Anti-ATG4D-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet, ATG4D in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für ATG4D Antikörper (C-Term) (ABIN1944848)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7567

Produktdetails anti-ATG4D Antikörper

Bindungsspezifität: AA 441-470, C-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-470 amino acids from the C-terminal region of human ATG4D.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-ATG4D Antikörper (ABIN1944848)

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Melvin: "Make Milwaukee safe for babies: the Child Welfare Commission and the development of urban health centers, 1911-1912." in: Journal of the West, Vol. 17, Issue 2, pp. 83-93, (1980) (PubMed).

Pontén, Westermark: "Properties of human malignant glioma cells in vitro." in: Medical biology, Vol. 56, Issue 4, pp. 184-93, (1978) (PubMed).

Westermark, Pontén, Hugosson: "Determinants for the establishment of permanent tissue culture lines from human gliomas." in: Acta pathologica et microbiologica Scandinavica. Section A, Pathology, Vol. 81, Issue 6, pp. 791-805, (1974) (PubMed).

Details zu ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Andere Bezeichnung: ATG4D

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Molekulargewicht: 52922

Gen-ID: 84971

NCBI Accession: NP_001268433, NP_116274

UniProt: Q86TL0

Pathways: Autophagie