SOD1 Antikörper (Internal Region)

Produktnummer ABIN190907

Der Ziege Polyklonal Anti-SOD1-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, SOD1 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SOD1 Antikörper (Internal Region) (ABIN190907)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-SOD1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: SOD1

Sequenz: SRKHGGPKDE ERH

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mous

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells and the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-SOD1 Antikörper (ABIN190907)

Frazziano, Al Ghouleh, Baust, Shiva, Champion, Pagano: "Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 2, pp. H197-205, (2014) (PubMed).

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) , HGNC:11179, ALS, ALS1, IPOA, SOD , Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154), superoxide

Gen-ID: 6647, 20655, 24786

NCBI Accession: NP_000445

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis