LHCGR Antikörper (C-Term)

Produktnummer ABIN190901

Der Ziege Polyklonal Anti-LHCGR-Antikörper wurde für IF, FACS und ELISA validiert. Er ist geeignet, LHCGR in Proben von Human zu detektieren.

Kurzübersicht für LHCGR Antikörper (C-Term) (ABIN190901)

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser LHCGR Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Güteklasse: Verified

Produktdetails anti-LHCGR Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: LHCGR

Sequenz: CQGTALLDKT RYTE

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CQGTALLDKTRYTE, from the C Terminus of the protein sequence according to NP_000224.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave an approx 35 kDa band in K562 cell lysates after 0.1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please not

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu LHCGR

Target: LHCGR (Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR))

Andere Bezeichnung: LHCGR

Hintergrund: LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor

Gen-ID: 3973

NCBI Accession: NP_000224

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, cAMP Metabolic Process, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling