Nanog Antikörper (C-Term)

Produktnummer ABIN190835

Der Ziege Polyklonal Anti-Nanog-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, Nanog in Proben von Human zu detektieren.

Kurzübersicht für Nanog Antikörper (C-Term) (ABIN190835)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NANOG (C Terminus)

Sequenz: DLFLNYSMNM QPED

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. An additional band of approx. 37 kDa is observed and is consistent with the obser

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG

Hintergrund: NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Gen-ID: 79923

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance