RPA1 Antikörper

Produktnummer ABIN190714

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch RPA1 in WB, IP und ChIP. Es zeigt Reaktivität gegenüber Proben von Saccharomyces cerevisiae. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für RPA1 Antikörper (ABIN190714)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-RPA1 Antikörper

Verwendungszweck: Anti-RFA | Baker's yeast replication factor A

Kreuzreaktivität (Details): Saccharomyces cerevisiae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Saccharomyces cerevisiae

Aufreinigung: Serum

Immunogen: RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa)

Anwendungsinformationen

Applikationshinweise: ChIP, 1 : 20 000 (WB)

Kommentare:

antibody was also successfully used in ChIP application, presented data are courtesy of M. Pool and Dr. H. van Attikum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-RPA1 Antikörper (ABIN190714)

Chen, Lisby, Symington: "RPA coordinates DNA end resection and prevents formation of DNA hairpins." in: Molecular cell, Vol. 50, Issue 4, pp. 589-600, (2013) (PubMed).

Bentsen, Nielsen, Lisby, Nielsen, Gupta, Mundbjerg, Andersen, Bjergbaek: "MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context." in: Nucleic acids research, Vol. 41, Issue 5, pp. 3173-89, (2013) (PubMed).

Details zu RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Andere Bezeichnung: RFA

Hintergrund: Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody

Molekulargewicht: 70 + 30 + 14 kDa

Gen-ID: 851266

UniProt: P22336, P26754

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA