GCLC Antikörper (C-Term)

Produktnummer ABIN190704

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-GCLC Antikörper (ABIN190704) detektiert spezifisch GCLC in WB und IL. Dieser Antikörper reagiert spezifisch mit Proben aus Mais (Zea mays), Tomato, Salicornia, Nicotiana tabacum und Arabidopsis thaliana.

Kurzübersicht für GCLC Antikörper (C-Term) (ABIN190704)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Mais (Zea mays), Tomato, Salicornia, Nicotiana tabacum, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-GCLC Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-Gamma-ECS | Gamma glutamylcysteine synthase

Spezifität: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Kreuzreaktivität (Details): Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Marchantia polymorpha,Pisum sativum,Riciuns communis,Oryza sativa,Triticum aestivum

Aufreinigung: Protein G purified

Immunogen: KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1 : 5000 (IL), 1 : 5000 (WB)

Kommentare:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-GCLC Antikörper (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: Gamma-ECS

Hintergrund:

Background: Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Additional information: Total IgG concentration is 5,2 μg/ μl

Molekulargewicht: 50 kDa

Gen-ID: 828409

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis