Pyruvate Dehydrogenase E1 alpha (AA 39-295) Antikörper

Produktnummer ABIN1886427

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB und IF. Geeignet für Human.

Kurzübersicht für Pyruvate Dehydrogenase E1 alpha (AA 39-295) Antikörper (ABIN1886427)

Target: Pyruvate Dehydrogenase E1 alpha

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: AA 39-295

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 39 and 295 of Pyruvate Dehydrogenase E1 alpha

Anwendungsinformationen

Applikationshinweise: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Biohazard Informations: This product contains thimerosal which is hazardous.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Antigendetails

Target: Pyruvate Dehydrogenase E1 alpha

Hintergrund: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA.The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4).The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits.The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]

Molekulargewicht: 43 kDa

Gen-ID: 5160

NCBI Accession: NM_000284, NP_000275