H2AFX Antikörper (C-Term)

Produktnummer ABIN1882250

Der Maus Monoklonal Anti-H2AFX-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, H2AFX in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für H2AFX Antikörper (C-Term) (ABIN1882250)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser H2AFX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 938CT5-1-1

Produktdetails anti-H2AFX Antikörper

Bindungsspezifität: AA 115-143, C-Term

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC: 1:50. IHC-P: 1:25. IHC-P: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-H2AFX Antikörper (ABIN1882250)

Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." in: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." in: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

Park, Chan, Park, Oettinger, Kwon: "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner." in: Nucleic acids research, Vol. 31, Issue 23, pp. 6819-27, (2003) (PubMed).

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: H2AFX

Hintergrund: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Molekulargewicht: 15145

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus