EIF3E Antikörper (AA 248-276)

Produktnummer ABIN1881290

Der Kaninchen Polyklonal Anti-EIF3E-Antikörper wurde für WB validiert. Er ist geeignet, EIF3E in Proben von Human und Zebrafisch (Danio rerio) zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für EIF3E Antikörper (AA 248-276) (ABIN1881290)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB40515

Produktdetails anti-EIF3E Antikörper

Bindungsspezifität: AA 248-276

Homologie: X, B, C, Pr, M, Rat

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-EIF3E Antikörper (ABIN1881290)

Grzmil, Rzymski, Milani, Harris, Capper, Saunders, Salhan, Ragoussis, Norbury: "An oncogenic role of eIF3e/INT6 in human breast cancer." in: Oncogene, Vol. 29, Issue 28, pp. 4080-9, (2010) (PubMed).

Zhou, Sandercock, Fraser, Ridlova, Stephens, Schenauer, Yokoi-Fong, Barsky, Leary, Hershey, Doudna, Robinson: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, (2008) (PubMed).

Morris, Wittmann, Jäck, Jalinot: "Human INT6/eIF3e is required for nonsense-mediated mRNA decay." in: EMBO reports, Vol. 8, Issue 6, pp. 596-602, (2007) (PubMed).

Masutani, Sonenberg, Yokoyama, Imataka: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, (2007) (PubMed).

Sirchia, Luparello: "Mid-region parathyroid hormone-related protein (PTHrP) and gene expression of MDA-MB231 breast cancer cells." in: Biological chemistry, Vol. 388, Issue 5, pp. 457-65, (2007) (PubMed).

Details zu EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Andere Bezeichnung: EIF3E

Hintergrund: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD), may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Molekulargewicht: 52221

NCBI Accession: NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C