This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-290 amino acids from the Central region of human ATG4B.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Kabeya, Mizushima, Yamamoto, Oshitani-Okamoto, Ohsumi, Yoshimori: "LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation." in: Journal of cell science, Vol. 117, Issue Pt 13, pp. 2805-12, (2004) (PubMed).
Target
ATG4B
(Autophagy related 4B Cysteine Peptidase (ATG4B))
Andere Bezeichnung
ATG4B
Hintergrund
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.