APAF1 Antikörper

Produktnummer ABIN187407

Der Ratte Monoklonal anti-APAF1 Antikörper (Klon 2E12) (ABIN187407) detektiert spezifisch APAF1 in WB, IP, IF und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für APAF1 Antikörper (ABIN187407)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 2E12

Produktdetails anti-APAF1 Antikörper

Spezifität: Recognizes the CARD domain of human Apaf-1. Species Reactivity: Human, others not tested. Does not cross-react with mouse or rat Apaf-1.

Immunogen: FLAG-tagged recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry Immunohistochemistry: Electron microscopy on frozen sections. Immunoprecipitation Western blot ELISA The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL.

Buffer: 100 µg of liquid Protein G purified monoclonal antibody in PBS containing 0.02% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Andere Bezeichnung: APAF1

Hintergrund: Programmed cell death (apoptosis) requires the activation of caspases, which are in turn regulated by adaptor proteins such as Apaf-1 (activator of apoptosis protease-activating factor-1). The 136 kDa protein Apaf-1 is a mammalian homolog of C. elegans/CED-4, and is required for some pathways of cell death. Apaf-1 promotes the aggregation and self-processing of the initiator caspase, caspase-9 in the presence of cytochrome c and dATP. It consists of four function-determining regions: (1) an N-terminal caspase activation and recruitment domain (CARD), which is required for binding to pro-caspase-9, (2) a central CED-4 homologous region, (3) an Apaf-1 specific region, and (4) 12 identified WD40 repeats at the C-terminus of the protein.

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity