SMAD2 Antikörper (AA 272-467)

Produktnummer ABIN1860588

Dieses Anti-SMAD2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMAD2 in WB, IHC, IP und ICC. Geeignet für Ratte.

Kurzübersicht für SMAD2 Antikörper (AA 272-467) (ABIN1860588)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-SMAD2 Antikörper

Bindungsspezifität: AA 272-467

Verwendungszweck: Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)

Sequenz: MGHHHHHHSG SEF- AFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS

Spezifität: The antibody is a rabbit polyclonal antibody raised against Smad2. It has been selected for its ability to recognize Smad2 in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Smad2 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against Smad2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: Smad2

Hintergrund: JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus