Nephrin Antikörper (AA 27-234)

Produktnummer ABIN1860039

Dieses Anti-Nephrin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Nephrin in WB, IHC, IP und ICC. Geeignet für Human.

Kurzübersicht für Nephrin Antikörper (AA 27-234) (ABIN1860039)

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nephrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Nephrin Antikörper

Bindungsspezifität: AA 27-234

Verwendungszweck: Polyclonal Antibody to Nephrin (NPHN)

Spezifität: The antibody is a rabbit polyclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nephrin (NPHN) corresdonding to Pro27~Val234 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Andere Bezeichnung: NPHN

Hintergrund: NPHS1, CNF, Nephrosis 1, Congenital Finnish Type, Renal glomerulus-specific cell adhesion receptor

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development