CHI3L1 Antikörper (AA 112-377) (FITC)

Produktnummer ABIN1859043

Produktdetails anti-CHI3L1 Antikörper (FITC)

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Bindungsspezifität: AA 112-377

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHI3L1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Sequenz: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-NTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF TTLIKEMKAE FIKEAQPGKK QLLLSAALSA GKVTIDSSYD IAKISQHLDF ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA VGYMLRLGAP ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM VWALDLDDFQ GSFCGQDLRF PLTNAIK

Spezifität: It has been selected for its ability to recognize GP39 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant GP39 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against GP39 conjugated to fitc.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant GP39 (Asn112~Lys377) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu CHI3L1

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Andere Bezeichnung: Glycoprotein 39, Cartilage (GP39) (CHI3L1 Produkte)

Substanzklasse: Viral Protein