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alpha Fetoprotein Antikörper (AA 31-171) (FITC)

Dieses Anti-alpha Fetoprotein-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von alpha Fetoprotein in WB, IHC, IF und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN1857933

Kurzübersicht für alpha Fetoprotein Antikörper (AA 31-171) (FITC) (ABIN1857933)

Target

Alle alpha Fetoprotein (AFP) Antikörper anzeigen
alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität

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Human

Wirt

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Kaninchen

Klonalität

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Polyklonal

Konjugat

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Dieser alpha Fetoprotein Antikörper ist konjugiert mit FITC

Applikation

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 16
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    • 1
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    • 1
    • 1
    AA 31-171

    Verwendungszweck

    FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

    Sequenz

    MGHHHHHHSG SEF- ILDSYQCTAE ISLADLATIF FAQFVQEATY KEVSKMVKDA LTAIEKPTGD EQSSGCLENQ LPAFLEELCH EKEILEKYGH SDCCSQSEEG RHNCFLAHKK PTPASIPLFQ VPEPVTSCEA YEEDRETFMN KFIYEIARRH P

    Spezifität

    The antibody is a rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in immunohistochemical staining and western blotting.

    Aufreinigung

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    Recombinant aFP expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against aFP conjugated to fitc.

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handhabung

    Avoid repeated freeze/thaw cycles

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Yang, Chen, Wang, Xia, Cheng, Pei, Wang, Bao: "Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip." in: Biosensors & bioelectronics, Vol. 48, pp. 153-7, (2013) (PubMed).

  • Target

    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

    Andere Bezeichnung

    Alpha-Fetoprotein

    Hintergrund

    AFP, A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein

    Pathways

    C21-Steroid Hormone Metabolic Process
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