Melanoma gp100 Antikörper (C-Term)

Produktnummer ABIN185660

Der Ziege Polyklonal Anti-Melanoma gp100-Antikörper wurde für WB, IF und ELISA validiert. Er ist geeignet, Melanoma gp100 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Melanoma gp100 Antikörper (C-Term) (ABIN185660)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Güteklasse: Verified

Produktdetails anti-Melanoma gp100 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Silver homologue / Pmel 17 (C-terminus)

Sequenz: CPIGENSPLL SGQQ

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in Mouse Skin lysates (calculated MW of 66.3 kDa according to Mouse NP_068682.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of A431cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: PMEL

Hintergrund: Silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog

Gen-ID: 6490, 20431, 362818

NCBI Accession: NP_008859