Leptin Receptor Antikörper (Internal Region)

Produktnummer ABIN185639

Der Ziege-Polyklonal Anti-Leptin Receptor-Antikörper wurde für WB, ELISA, IHC und FACS validiert. Er ist geeignet zum Nachweis von Leptin Receptor in Proben aus Human, Maus und Ratte. }

Kurzübersicht für Leptin Receptor Antikörper (Internal Region) (ABIN185639)

Target: Leptin Receptor (LEPR)

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Leptin Receptor Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Leptin Receptor

Sequenz: TQDDIEKHQS DAG

Spezifität: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2, NP_001003679.1, NP_001003680.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140-150 kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132 kDa according to Human NP_002294.2 and 130.8 kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: LEPR

Hintergrund: LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB

Gen-ID: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour