Leptin Receptor Antikörper (Internal Region)

Produktnummer ABIN185639

Der Ziege Polyklonal Anti-Leptin Receptor-Antikörper wurde für WB, ELISA, IHC und FACS validiert. Er ist geeignet, Leptin Receptor in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Leptin Receptor Antikörper (Internal Region) (ABIN185639)

Target: Leptin Receptor (LEPR)

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Leptin Receptor Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Leptin Receptor

Sequenz: TQDDIEKHQS DAG

Spezifität: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2, NP_001003679.1, NP_001003680.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140-150 kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132 kDa according to Human NP_002294.2 and 130.8 kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Andere Bezeichnung: LEPR

Hintergrund: LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB

Gen-ID: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour