Choline Acetyltransferase Antikörper (C-Term)

Produktnummer ABIN185635

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Choline Acetyltransferase in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Choline Acetyltransferase Antikörper (C-Term) (ABIN185635)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-Choline Acetyltransferase Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Chat (mouse)

Sequenz: KERARGPTKP SNLD

Spezifität: This antibody is NOT expected to cross-react to Human CHAT.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.

Western Blot: Approx 75 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: Chat

Hintergrund: Chat, choline acetyltransferase , B230380D24Rik

Gen-ID: 12647, 432359

NCBI Accession: NP_034021

Pathways: Skeletal Muscle Fiber Development