PGRMC1 Antikörper (C-Term)

Produktnummer ABIN185610

Dieses Anti-PGRMC1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von PGRMC1 in WB, IHC, ELISA und IF. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PGRMC1 Antikörper (C-Term) (ABIN185610)

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PGRMC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-PGRMC1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PGRMC1 / MPR

Sequenz: EPKDESARKN D

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EPKDESARKND, from the C Terminus of the protein sequence according to NP_006658.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 24 kDa band observed in Human Kidney, Liver and Testis lysates, and approx 25 kDa in Mouse Testis lysates (calculated MW of 21.7 kDa according to Human NP_006658.1 and Mouse NP_058063.2). These molecular weights are routinely observed b

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: This antibody has been successfully used in IF on Human: Izzo, N. J et al. (2014) PMID: 25390692.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PGRMC1 Antikörper (ABIN185610)

Izzo, Xu, Zeng, Kirk, Mozzoni, Silky, Rehak, Yurko, Look, Rishton, Safferstein, Cruchaga, Goate, Cahill, Arancio, Mach, Craven, Head, LeVine, Spires-Jones, Catalano: "Alzheimer's therapeutics targeting amyloid beta 1-42 oligomers II: Sigma-2/PGRMC1 receptors mediate Abeta 42 oligomer binding and synaptotoxicity." in: PLoS ONE, Vol. 9, Issue 11, pp. e111899, (2015) (PubMed).

Details zu PGRMC1

Target: PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))

Andere Bezeichnung: PGRMC1

Hintergrund: PGRMC1, MPR, progesterone receptor membrane component 1 , HGNC:16090, HPR6.6, progesterone binding protein

Gen-ID: 10857, 53328

NCBI Accession: NP_006658