LEF1 Antikörper (Internal Region)

Produktnummer ABIN185607

Dieses Anti-LEF1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von LEF1 in WB, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für LEF1 Antikörper (Internal Region) (ABIN185607)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-LEF1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: LEF1

Sequenz: QHEQRKEQEP KRPH

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 50--55 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 44.2 kDa according to NP_057353.1)/ This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the imm

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: LEF1

Hintergrund: LEF1, lymphoid enhancer-binding factor 1 , HGNC:6551, DKFZp586H0919, TCF1ALPHA , lymphoid enhancer binding factor-1

Gen-ID: 51176, 16842, 161452

NCBI Accession: NP_057353

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding