ADRB1 Antikörper (Internal Region)

Produktnummer ABIN185584

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ADRB1 in ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für ADRB1 Antikörper (Internal Region) (ABIN185584)

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ADRB1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ADRB1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ADRB1

Sequenz: ESDEARRCYN DPK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ESDEARRCYNDPK, from the internal region of the protein sequence according to NP_000675.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 6 μg/mL.

Western Blot: This antibody is not recommended for use in Western blotting.

Peptide ELISA: antibody detection limit dilution 1:2000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plama membrane and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ADRB1 Antikörper (ABIN185584)

Wenzel, Schulze-Rothe, Müller, Wallukat, Haberland: "Difference between beta1-adrenoceptor autoantibodies of human and animal origin-Limitations detecting beta1-adrenoceptor autoantibodies using peptide based ELISA technology." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192615, (2018) (PubMed).

Wallukat, Prüss, Müller, Schimke: "Functional autoantibodies in patients with different forms of dementia." in: PLoS ONE, Vol. 13, Issue 3, pp. e0192778, (2018) (PubMed).

Wenzel, Schulze-Rothe, Haberland, Müller, Wallukat, Davideit: "Performance and in-house validation of a bioassay for the determination of beta1-autoantibodies found in patients with cardiomyopathy." in: Heliyon, Vol. 3, Issue 7, pp. e00362, (2017) (PubMed).

Wallukat, Haberland, Berg, Schulz, Freyse, Dahmen, Kage, Dandel, Vetter, Salzsieder, Kreutz, Schimke: "The first aptamer-apheresis column specifically for clearing blood of ?1-receptor autoantibodies." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 76, Issue 10, pp. 2449-55, (2012) (PubMed).

Haberland, Wallukat, Dahmen, Kage, Schimke: "Aptamer neutralization of beta1-adrenoceptor autoantibodies isolated from patients with cardiomyopathies." in: Circulation research, Vol. 109, Issue 9, pp. 986-92, (2011) (PubMed).

Wallukat, Muñoz Saravia, Haberland, Bartel, Araujo, Valda, Duchen, Diaz Ramirez, Borges, Schimke: "Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients." in: Journal of the American College of Cardiology, Vol. 55, Issue 5, pp. 463-8, (2010) (PubMed).

Details zu ADRB1

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Andere Bezeichnung: ADRB1

Hintergrund: ADRB1, adrenergic, beta-1-, receptor, ADRB1R, B1AR, BETA1AR, RHR, beta-1-adrenergic receptor

Molekulargewicht: 51.2kDa

Gen-ID: 153

NCBI Accession: NP_000675

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation